![]() ![]() OspC expression is maintained early during infection. During tick feeding, the bacteria leave the tick’s mid gut and express OspC on their surface. burgdorferi regulates its outer surface protein expression depending on its environment. burgdorferi antigens in a single sample 13.įigure 2: B. The test is based on fluorescent beads and allows the simultaneous measurement of antibodies to all three B. It detects antibodies to three antigens of B. The Equine Lyme Multiplex Assay was developed at the Animal Health Diagnostic Center at Cornell University. How does the Equine Lyme Multiplex Assay work? burgdorferi by three to five weeks after infection 12. The Equine Lyme Multiplex Assay can identify antibodies to B. These assays detected antibodies as early as five to six weeks after infection and resulted in positive antibody titers by ten to twelve weeks in most experimentally infected ponies 11. The assay values are expressed as median fluorescent intensities (MFI).ĭiagnostic Lyme antibody testing in horse serum has been performed by ELISA followed by a confirmatory Western blot or by immunofluorescence assays (IFA). Outer surface protein (Osp) specific antibodies in the horse’s serum bind to OspA, OspC or OspF multiplex beads, are detected by a fluorescent conjugate and are evaluated in a multiplex reader. orthopedic disease, behavioral or training issuesįigure 1: The Equine Lyme Multiplex Assay detects antibodies to B. Ruling out other causes of clinical signs that may have similar signs than those associated with Lyme disease, i.e. Horses that have a history of visiting an area with infected ticksĬlinical signs compatible with Lyme disease The diagnosis of Lyme disease in horses 10 can be made based on: ![]() Most recent reports describe horses with a Borrelia-associated pseudolymphoma 8 or Borrelia-associated uveitis 9. Neurological signs such as depression, dysphagia, head tilt and encephalitis were reported in chronic cases 2,6,7. Changes in behavior and skin sensitivity (tactile hyper-aesthesia), both with rapid onset, are common clinical signs seen by many practitioners in horses with potential Lyme disease. If clinical signs occur, they can include chronic weight loss, sporadic lameness, shifting leg lameness, low-grade fever, muscle tenderness, chronically poor performance, swollen joints, arthritis and diverse orthopedic problems 2-5. Not all infected horses develop clinical signs of Lyme disease. Similar to humans, horses are incidental, dead-end hosts for B. burgdorferi. Spirochetes are transmitted to horses by infected ticks. Lyme disease is induced by the spirochete B. ![]()
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